Construction of a pilot human YAC library in a recombination-defective yeast strain.

doi:10.1016/s0378-1119(96)00751-2

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Article

Construction of a pilot human YAC library in a recombination-defective yeast strain.

Gene - 1 Apr 1997

Palmieri G, Miano M G, Casamassimi A, Lania A, Kohno K, Schlessinger D, D'Urso M, Featherstone T

Abstract excerpt

Using high-molecular-weight DNA fragments from a human lymphoblastoid cell line, a pilot collection of 2500 YACs was constructed in YKK115, a recombination-deficient strain of Saccharomyces cerevisiae carrying mutations in both the rad51 and rad52 genes. Analysis of 520 clones from the current li...

Topics

Cell Line
Chromosome Mapping
Chromosomes, Artificial, Yeast
DNA-Binding Proteins
Fungal Proteins
Gene Library
Humans
In Situ Hybridization, Fluorescence
Mutation
Rad51 Recombinase
Rad52 DNA Repair and Recombination Protein
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transformation, Genetic

Source

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Identifiers

PMID: 9133588
DOI: 10.1016/s0378-1119(96)00751-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9133588-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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