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Article

Construction of a pilot human YAC library in a recombination-defective yeast strain.

Gene - 1 Apr 1997

Palmieri G, Miano M G, Casamassimi A, Lania A, Kohno K, Schlessinger D, D'Urso M, Featherstone T

Abstract excerpt

Using high-molecular-weight DNA fragments from a human lymphoblastoid cell line, a pilot collection of 2500 YACs was constructed in YKK115, a recombination-deficient strain of Saccharomyces cerevisiae carrying mutations in both the rad51 and rad52 genes. Analysis of 520 clones from the current library by pulsed-field gel electrophoresis revealed more than 97% single YACs with an insert size averaging 340 kb....

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Identifiers

PMID: 9133588
DOI: 10.1016/s0378-1119(96)00751-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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