Article
Construction and characterization of a rad51rad52 double mutant as a host for YAC libraries.
Gene - 1 Apr 1997
Kohno K, Oshiro T, Kishine H, Wada M, Takeda H, Ihara N, Imamoto F, Kano Y, Schlessinger D
Abstract excerpt
RAD52 or RAD51 recombination-deficient yeast strains stabilize otherwise unstable YACs containing ribosomal DNA or the human color vision locus (Kohno et al., 1994). Thus the RAD52RAD51 pathways(s) are apparently involved in the instability of YACs containing tandem repeat loci, presumably by pro...
Topics
- Chromosomes, Artificial, Yeast
- DNA-Binding Proteins
- Fungal Proteins
- Gene Library
- Humans
- Mutation
- Plasmids
- Rad51 Recombinase
- Rad52 DNA Repair and Recombination Protein
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Species Specificity