Article
Analysis of mammalian 20S proteasome biogenesis: the maturation of beta-subunits is an ordered two-step mechanism involving autocatalysis.
The EMBO journal - 16 Dec 1996
Schmidtke G, Kraft R, Kostka S, Henklein P, Frömmel C, Löwe J, Huber R, Kloetzel P M, Schmidt M
Abstract excerpt
Maturation of eukaryotic 20S proteasomes involves the processing of beta-subunits by limited proteolysis. To study the processing mechanism we analysed different point mutations of the beta-subunit LMP2 in transfected human T2 cells. Here we show that the presence of the intact Gly-1Thr1 consensu...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Biological Evolution
- Catalysis
- Cysteine Endopeptidases
- Humans
- Molecular Sequence Data
- Multienzyme Complexes
- Mutagenesis, Site-Directed
- Mutation
- Peptide Hydrolases
- Proteasome Endopeptidase Complex
- Protein Processing, Post-Translational