Article
Critical elements in proteasome assembly.
Nature structural biology - 1 Nov 1994
Zwickl P, Kleinz J, Baumeister W
Abstract excerpt
Coexpression of both subunits of the Thermoplasma proteasome in Escherichia coli yields fully assembled and proteolytically active proteasomes. Post-translational processing of the beta-subunit occurs in E. coli as it does in Thermoplasma. Coexpression of the alpha-subunit and the beta delta pro-subunit, a mutant beta-subunit lacking the propeptide, also yields fully assembled and active proteasomes. This...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Cysteine Endopeptidases
- Enzyme Precursors
- Escherichia coli
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Proteasome Endopeptidase Complex
- Protein Conformation
- Protein Folding