Article
Critical elements in proteasome assembly.
Nature structural biology - 1 Nov 1994
Zwickl P, Kleinz J, Baumeister W
Abstract excerpt
Coexpression of both subunits of the Thermoplasma proteasome in Escherichia coli yields fully assembled and proteolytically active proteasomes. Post-translational processing of the beta-subunit occurs in E. coli as it does in Thermoplasma. Coexpression of the alpha-subunit and the beta delta pro-subunit, a mutant beta-subunit lacking the propeptide, also yields fully assembled and active proteasomes. This...
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