Article
Expression of Escherichia coli recA and ada genes in Saccharomyces cerevisiae using a vector with geneticin resistance.
Folia microbiologica - 1 Jan 1995
Slaninová M, Farkasová E, Chovanec M, Vlcková V, Näslund M, Henriques J A, Brozmanová J
Abstract excerpt
Construction of E. coli-yeast shuttle plasmids containing the neo selection gene is described. The protein-coding regions of the E. coli ada or recA genes under the control of the ADH1 promoter and terminator were ligated into the SphI unique site of pNF2 to produce pMSada and pMSrecA, respective...
Topics
- Bacterial Proteins
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Genes, Bacterial
- Genetic Vectors
- Genotype
- Gentamicins
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase