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Article

Expression of Escherichia coli recA and ada genes in Saccharomyces cerevisiae using a vector with geneticin resistance.

Folia microbiologica - 1 Jan 1995

Slaninová M, Farkasová E, Chovanec M, Vlcková V, Näslund M, Henriques J A, Brozmanová J

Abstract excerpt

Construction of E. coli-yeast shuttle plasmids containing the neo selection gene is described. The protein-coding regions of the E. coli ada or recA genes under the control of the ADH1 promoter and terminator were ligated into the SphI unique site of pNF2 to produce pMSada and pMSrecA, respectively. The plasmids were used for transformation of the haploid and diploid pso4-1 strains of S. cerevisiae and their...

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PMID: 8919931
DOI: 10.1007/BF02814203

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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