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Article

The Escherichia coli recA gene increases resistance of the yeast Saccharomyces cerevisiae to ionizing and ultraviolet radiation.

Molecular & general genetics : MGG - 1 Jul 1991

Brozmanová J, Cernáková L, Vlcková V, Duraj J, Fridrichová I

Abstract excerpt

The Escherichia coli recA protein coding region was ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase promoter region to produce plasmid pADHrecA. Transformation of the wild-type yeast strains YNN-27 and 7799-4B, as well as the recombination-deficient rad52-1 C5-6 mutant, with this shuttle plasmid resulted in the expression of the bacterial 38 kDa...

Topics

Alcohol Dehydrogenase
DNA, Fungal
Escherichia coli
Genes, Bacterial
Genes, Fungal
Haploidy
Mutation
Plasmids
Promoter Regions, Genetic
Rec A Recombinases
Restriction Mapping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae

Transformation, Genetic

Ultraviolet Rays

Source

Identifiers

PMID: 1865881
DOI: 10.1007/BF00273940

Genes

RAD52

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1865881-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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