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Article

Construction of an Azospirillum brasilense Sp7 recA mutant.

Molecular & general genetics : MGG - 1 Aug 1990

Croes C, Vanderleyden J, Michiels K

Abstract excerpt

Cosmid clones encoding the recA gene of Azospirillum brasilense were isolated by intergeneric complementation of an Escherichia coli recA mutant. Site-directed Tn5 mutagenesis and subcloning of one complementing cosmid clone allowed us to localize the A. brasilense recA gene on a 1.2 kb DNA fragment. One Tn5 insertion that inactivates the cloned recA gene was crossed into the chromosome of A. brasilense by marker...

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Source

Identifiers

PMID: 2175386
DOI: 10.1007/BF00315808

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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