Construction of an Azospirillum brasilense Sp7 recA mutant.

doi:10.1007/BF00315808

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Article

Construction of an Azospirillum brasilense Sp7 recA mutant.

Molecular & general genetics : MGG - 1 Aug 1990

Croes C, Vanderleyden J, Michiels K

Abstract excerpt

Cosmid clones encoding the recA gene of Azospirillum brasilense were isolated by intergeneric complementation of an Escherichia coli recA mutant. Site-directed Tn5 mutagenesis and subcloning of one complementing cosmid clone allowed us to localize the A. brasilense recA gene on a 1.2 kb DNA fragm...

Topics

Azospirillum brasilense
Cloning, Molecular
Cosmids
DNA Transposable Elements
Genes, Bacterial
Mutation
Rec A Recombinases
Recombination, Genetic
Restriction Mapping
Ultraviolet Rays

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