Expression of the Escherichia coli recA gene in the yeast Saccharomyces cerevisiae.

doi:10.1016/0300-9084(91)90215-m

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Article

Expression of the Escherichia coli recA gene in the yeast Saccharomyces cerevisiae.

Biochimie - 1 Jan 2000

Cernakova L, Fridrichova I, Pirsel M, Kleibl K, Duraj J, Brozmanova J

Abstract excerpt

The isolation of the protein coding region of the recA gene from Escherichia coli by extensive Bal31 digestion is described. The structural recA gene was ligated into an extrachromosomally replicating yeast expression vector, downstream of the yeast alcohol-dehydrogenase gene promoter region, to...

Topics

Alcohol Dehydrogenase
Cloning, Molecular
DNA Repair
Escherichia coli
Gene Expression
Mutation
Plasmids
Promoter Regions, Genetic
Rec A Recombinases
Saccharomyces cerevisiae
Transfection
Transformation, Genetic

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