Article
[A system for analysis of yeast mutants deficient in the genetic recombination process].
Genetika - 1 Jul 1992
Korolev V G, Kozhinov T N, Kozhin S A, Peshekhonov V T, Chepurpaia O V
Abstract excerpt
A system of molecular-genetic methods intended for analysis of yeast Rec-mutants was developed. A number of plasmids containing noncomplementing mutations in the ADE2 gene and different selective markers were constructed. The system was based upon the phenomenon of interplasmid intragenic recombination during transformation and mitotic division. Transformation of yeast cells with the plasmid containing two long...
Topics
- Genes, Fungal
- Mitosis
- Models, Genetic
- Mutation
- Plasmids
- Recombination, Genetic
- Saccharomyces cerevisiae
- Transformation, Genetic