The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosaccharomyces pombe his5-303 and lys1-131 mutations, respectively: new selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and pSP4.

Abstract excerpt

Three new S. pombe plasmids are described. Plasmids pSP3 and pSP4 are two Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae HIS3 or LYS2 genes as selectable markers. They complement the S. pombe his5-303 or lys1-131 mutations, respectively. Plasmid pSPars1 is a ve...

Topics

• Aldehyde Oxidoreductases
• Fungal Proteins
• Gene Dosage
• Genes, Fungal
• Genetic Complementation Test
• Genetic Markers
• Genetic Vectors
• Genotype
• Hydro-Lyases
• L-Aminoadipate-Semialdehyde Dehydrogenase
• Mutation