Article
Investigation of a phage resistant Serratia entomophila strain (BC4B), establishment of generalised transduction and construction of S. entomophila RecA mutants.
Molecular & general genetics : MGG - 25 Feb 1996
Grkovic S, Mahanty H K
Abstract excerpt
A recA clone was isolated from a cosmid library of Serratia entomophila constructed in the Escherichia coli strain HB101. Subcloning and transposon mutagenesis were used to identify a 1.36 kb fragment containing the recA gene. A cloned recA mutation, generated by transposon mutagenesis and the re...
Topics
- Bacteriophages
- Cloning, Molecular
- DNA Transposable Elements
- Drug Resistance, Microbial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genotype
- Methyl Methanesulfonate
- Mutagenesis
- Mutagens
