Article
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
Journal of bacteriology - 1 Apr 1998
Murphy K C
Abstract excerpt
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the s...
Topics
- Bacteriophage lambda
- Calcium
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Primers
- DNA, Superhelical
- Escherichia coli
- Gene Targeting
- Genotype
- Operon
- Plasmids
- Polymerase Chain Reaction
- Recombinant Fusion Proteins
- Recombination, Genetic
- Restriction Mapping