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Article

Evaluation of Escherichia coli recBC sbcBC mutants for cloning by recombination in vivo.

Journal of biotechnology - 15 Apr 1995

Degryse E

Abstract excerpt

In vivo recombination as a tool for plasmid construction was analyzed using a model system based on the properties of the RecF pathway in Escherichia coli. This pathway was used after a double strand break (DSB) effected by restriction enzyme cleavage of the plasmids of interest. DSB repair was shown to be independent of the methylation state of the vector or insert DNA, of the type of restriction enzyme used (5'...

Topics

Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA Repair
Deoxyribonucleases
Escherichia coli
Escherichia coli Proteins
Exodeoxyribonuclease V
Exodeoxyribonucleases
Genes, Bacterial
Methylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Plasmids

Recombination, Genetic

Templates, Genetic

Source

Identifiers

PMID: 7755971
DOI: 10.1016/0168-1656(95)00019-m

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7755971-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Evaluation of Escherichia coli recBC sbcBC mutants for cloning by recombination in vivo.PMID 7755971Read ↗

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