Article
Molecular cloning and functional characterization of a recA analog from Pseudomonas stutzeri and construction of a P. stutzeri recA mutant.
Antonie van Leeuwenhoek - 1 Feb 1991
Vosman B, Hellingwerf K J
Abstract excerpt
A recombinant plasmid carrying the Pseudomonas stutzeri recA gene was isolated by complementation of the Escherichia coli recA13 mutation. Subcloning experiments showed that the gene was located on a 1500 bp PvuII-BglII fragment. The cloned gene complements an E. coli recA mutant for resistance t...
Topics
- Chromosome Deletion
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Methyl Methanesulfonate
- Mutagenesis
- Pseudomonas
- Rec A Recombinases
- Recombinant Proteins
- Recombination, Genetic
- Restriction Mapping
- Ultraviolet Rays