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Article

Use of the yeast one-hybrid system to screen for mutations in the ligand-binding domain of the estrogen receptor.

Steroids - 1 Mar 1996

Bush S M, Folta S, Lannigan D A

Abstract excerpt

This report describes a novel yeast one-hybrid system which easily allows for the detection of mutations in the ligand-binding domain of the estrogen receptor. This screen is based on the observation that a fusion protein consisting of the GAL4 DNA-binding domain and the estrogen receptor can interact with a GAL4 upstream activating sequence and induce the expression of an integrated GAL1-lacZ gene only in the...

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Identifiers

PMID: 8852826
DOI: 10.1016/0039-128x(95)00200-a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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