Article
Random mutagenesis of human estrogen receptor ligand binding domain identifies mutations that decrease sensitivity to estradiol and increase sensitivity to a diphenol indene-ol compound: basis for a regulatable expression system.
The Journal of steroid biochemistry and molecular biology - 1 Feb 1998
Miller N, Whelan J
Abstract excerpt
We have used low fidelity polymerase chain reaction amplification to generate mutations in the human estrogen receptor ligand binding domain (LBD). Screening of libraries of mutants in yeast revealed a variety of phenotypic changes including decreased responsiveness to estradiol and increased res...
Topics
- Amino Acid Sequence
- Binding Sites
- Estradiol
- Gene Expression Regulation
- Genes, Reporter
- Humans
- Indenes
- Ligands
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis
- Mutation
- Phenols
- Phenotype
- Polymerase Chain Reaction
- Receptors, Estrogen
- Saccharomyces cerevisiae
- Substrate Specificity