Article
Characterization of a temperature-sensitive mutation in the hormone binding domain of the human estrogen receptor. Studies in cell extracts and intact cells and their implications for hormone-dependent transcriptional activation.
The Journal of biological chemistry - 15 May 1992
Reese J C, Katzenellenbogen B S
Abstract excerpt
A previous report from this laboratory (Reese, J.C., and Katzenellenbogen, B. S. (1991) J. Biol. Chem. 266, 10880-10887) identified an estrogen receptor (ER) mutant which had a similar binding affinity for estradiol as wild-type ER but displayed a dose-response shift for estradiol in transactivat...
Topics
- Alanine
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- CHO Cells
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Cricetinae
- Cysteine
- Estradiol
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Receptors, Estrogen
- Recombinant Fusion Proteins