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Article

Fast large-scale purification of tetracycline repressor variants from overproducing Escherichia coli strains.

Journal of chromatography. A - 23 Aug 1996

Ettner N, Müller G, Berens C, Backes H, Schnappinger D, Schreppel T, Pfleiderer K, Hillen W

Abstract excerpt

We constructed a plasmid for overexpression of Tn10 Tet repressor (TetR) by placing a synthetic tetR gene under control of the Pc promoter. Active TetR is expressed up to 30% of the total soluble cell protein. A protocol containing anion-exchange, cation-exchange, and size-exclusion chromatography steps is described for the large-scale purification of milligram amounts of TetR in three days. Cation-exchange...

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Identifiers

PMID: 8817886
DOI: 10.1016/0021-9673(96)00232-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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