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Article

Construction of a tetracycline inducible expression vector and characterization of its use in Vibrio cholerae.

Plasmid - 1 Nov 2014

Bina X Renee, Wong Eileen A, Bina Thomas F, Bina James E

Abstract excerpt

We report the construction of a tetracycline inducible expression vector that allows regulated gene expression in the enteric pathogen Vibrio cholerae. The expression vector, named pXB300, contains the tetracycline regulatory elements from Tn10, a multiple cloning site downstream of the tetA promoter and operator sequences, a ColE1 origin of replication, a β-lactamase resistance gene for positive selection, and...

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Identifiers

PMID: 25451701
DOI: 10.1016/j.plasmid.2014.10.004
PMCID: PMC5467732

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Source receipt: pubmed, retrieved 7 Sept 2026.

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