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Article

Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity.

Journal of molecular biology - 20 Feb 1998

Helbl V, Hillen W

Abstract excerpt

The TetR PQ39 mutant exhibits a new recognition specificity for the tetO-4C operator, but the affinity is not sufficiently high for use in vivo. A stepwise selection of additional mutations by cassette mutagenesis with randomization of residues in the TetR alpha-helix-turn-alpha-helix motif (HTH) yielded mutant TetR EA37PQ39YM42 showing a similar affinity and increased specificity for tetO-4C as wild-type TetR...

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Identifiers

PMID: 9512703
DOI: 10.1006/jmbi.1997.1540

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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