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Article

Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity.

Journal of molecular biology - 20 Feb 1998

Helbl V, Hillen W

Abstract excerpt

The TetR PQ39 mutant exhibits a new recognition specificity for the tetO-4C operator, but the affinity is not sufficiently high for use in vivo. A stepwise selection of additional mutations by cassette mutagenesis with randomization of residues in the TetR alpha-helix-turn-alpha-helix motif (HTH)...

Topics

Amino Acid Sequence
Binding Sites
Escherichia coli
Helix-Turn-Helix Motifs
Models, Molecular
Molecular Sequence Data
Mutagenesis
Mutation
Operator Regions, Genetic
Protein Conformation
Repressor Proteins
Substrate Specificity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 9512703
DOI: 10.1006/jmbi.1997.1540

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9512703-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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