Article
Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Rennig Maja, Daley Daniel O, Nørholm Morten H H
Abstract excerpt
Strategies to select highly expressed variants of a protein coding sequence are usually based on trial-and-error approaches, which are time-consuming and expensive. We address this problem using translationally coupled antibiotic resistance markers. The system requires that the target gene can be fused at the 3'-end with a translational coupling element and an antibiotic resistance gene. Highly expressed target...
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