Article
Length changes in the joining segment between domains 5 and 6 of a group II intron inhibit self-splicing and alter 3' splice site selection.
Molecular and cellular biology - 1 Oct 1996
Boulanger S C, Faix P H, Yang H, Zhuo J, Franzen J S, Peebles C L, Perlman P S
Abstract excerpt
Domain 5 (D5) and domain 6 (D6) are adjacent folded hairpin substructures of self-splicing group II introns that appear to interact within the active ribozyme. Here we describe the effects of changing the length of the 3-nucleotide segment joining D5 to D6 [called J(56)3] on the splicing reaction...
Topics
- Base Sequence
- DNA, Fungal
- DNA, Mitochondrial
- Genetic Variation
- Introns
- Mitochondria
- Molecular Sequence Data
- Nucleic Acid Conformation
- Nucleic Acid Denaturation
- Oxygen Consumption
- Plasmids
- RNA Splicing
- RNA, Catalytic
- RNA, Fungal
- Saccharomyces cerevisiae