Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.

RNA (New York, N.Y.) - 1 Jul 2009

Mefford Melissa A, Staley Jonathan P

Abstract excerpt

During pre-mRNA splicing, the spliceosome must configure the substrate, catalyze 5' splice site cleavage, reposition the substrate, and catalyze exon ligation. The highly conserved U2/U6 helix I, which adjoins sequences that define the reactive sites, has been proposed to configure the substrate for 5' splice site cleavage and promote catalysis. However, a role for this helix at either catalytic step has not been...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenosine Triphosphatases
Share to Catalysis
Share to RNA, Fungal

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to RNA Helicases

Share to RNA Precursors

Share to RNA, Small Nuclear

Share to RNA Splicing

Share to RNA Splicing Factors

Source

Open in PubMed Open full text

Identifiers

PMID: 19458033
DOI: 10.1261/rna.1582609
PMCID: PMC2704075

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.PMID 19458033Read ↗

The conserved central domain of yeast U6 snRNA: importance of U2-U6 helix Ia in spliceosome assembly.Semantic proximity Read ↗

Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.Semantic proximity Read ↗

Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2.Semantic proximity Read ↗

The DEAH-box splicing factor Prp16 unwinds RNA duplexes in vitro.Semantic proximity Read ↗

Characterization of Cwc2, U6 snRNA, and Prp8 interactions destabilized by Prp16 ATPase at the transition between the first and second steps of splicing.Semantic proximity Read ↗

Interactions of the yeast U6 RNA with the pre-mRNA branch site.Semantic proximity Read ↗

Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.Semantic proximity Read ↗

Biochemical and Genetic Evidence Supports Fyv6 as a Second-Step Splicing Factor in <i>Saccharomyces cerevisiae</i>Semantic proximity Read ↗

Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.Semantic proximity Read ↗

Brr2p-mediated unwinding of U4/U6 is promoted by a mutually exclusive intra-molecular stem loop in U4 and involves destabilization of the 5’ stem-loop of U4Semantic proximity Read ↗

The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing.Semantic proximity Read ↗

Structural Insights into Branch Site Proofreading by Human SpliceosomeSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.