Article
Studies of point mutants define three essential paired nucleotides in the domain 5 substructure of a group II intron.
Molecular and cellular biology - 1 Aug 1995
Boulanger S C, Belcher S M, Schmidt U, Dib-Hajj S D, Schmidt T, Perlman P S
Abstract excerpt
Domain 5 (D5) is a highly conserved, largely helical substructure of group II introns that is essential for self-splicing. Only three of the 14 base pairs present in most D5 structures (A2.U33, G3.U32, and C4.G31) are nearly invariant. We have studied effects of point mutations of those six nucleotides on self-splicing and in vivo splicing of aI5 gamma, an intron of the COXI gene of Saccharomyces cerevisiae...
Topics
- Base Composition
- Base Sequence
- Conserved Sequence
- DNA Mutational Analysis
- Electron Transport Complex IV
- Introns
- Molecular Sequence Data
- Phenotype
- Point Mutation
- RNA Splicing
- RNA, Catalytic