Self-splicing of a Podospora anserina group IIA intron in vitro. Effects of 3'-terminal intron alterations on cleavage at the 5' and 3' splice site.

doi:10.1006/jmbi.1993.1309

Back to search

Article

Self-splicing of a Podospora anserina group IIA intron in vitro. Effects of 3'-terminal intron alterations on cleavage at the 5' and 3' splice site.

Journal of molecular biology - 5 Jun 1993

Schmidt U, Sägebarth R, Schmelzer C, Stahl U

Abstract excerpt

A shortened derivative of the group IIA intron from the mitochondrial cytochrome-c-oxidase subunit I gene (COI I1) of the ascomycete Podospora anserina can undergo self-splicing in vitro. When compared to self-splicing group IIB introns from yeast mitochondria (aI5c, bI1) the autocatalytic reacti...

Topics

Ascomycota
Base Sequence
Introns
Kinetics
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA Precursors
RNA Splicing
RNA, Fungal

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.