Mutations of the two-nucleotide bulge of D5 of a group II intron block splicing in vitro and in vivo: phenotypes and suppressor mutations.

Article

Mutations of the two-nucleotide bulge of D5 of a group II intron block splicing in vitro and in vivo: phenotypes and suppressor mutations.

RNA (New York, N.Y.) - 1 Nov 1996

Schmidt U, Podar M, Stahl U, Perlman P S

Abstract excerpt

Domain 5 (D5) is a highly conserved substructure of group II introns that is essential for catalysis of both steps of the splicing pathway. Here we studied the effects of mutations of the conserved 2-nt bulge in the binding and catalytic functions of D5 of intron aI5gamma of yeast mitochondrial D...

Topics

Base Sequence
DNA, Fungal
DNA, Mitochondrial
Genes, Fungal
Introns
Mutation
Nucleic Acid Conformation
Phenotype
RNA Splicing
RNA, Fungal
Saccharomyces cerevisiae
Suppression, Genetic

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