Article
[A simplified method of HLA-DR typing with the PCR method using the genomic DNA extracted from blood-absorbed filter].
Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology - 1 Jun 1996
Kabayama H
Abstract excerpt
The enzymatic amplification of specific DNA sequences by the polymerase chain reaction (PCR) has provided a new approach to genetic typing of HLA-class II region specificities. However, we found the existing PCR protocols to be adequate and/or time consuming, especially when large numbers of samples need to be processed. We have devised a simplified method which alleviates these problems. Genomic DNA prepared...
Topics
- Adolescent
- Alleles
- Base Sequence
- Child
- DNA
- Genome, Human
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction