An HLA-DR typing protocol using group-specific PCR-amplification followed by restriction enzyme digests.

Abstract excerpt

A simple PCR-based protocol for HLA-DR typing suitable for a routine practice is described. The method involves, first, a PCR amplification with seven different, group-specific (DR1, DR2, DR4, DR7, DR9, DR10, and DR3+5+6+8) primer-pairs, and second, typing of HLA-DR allele more exactly in DR1, DR2, DR4, and DR3+5+6+8 groups by digestion of PCR products with restriction enzymes distinguishing different HLA-DR...

Topics

• Alleles
• Base Sequence
• DNA
• DNA Restriction Enzymes
• Evaluation Studies as Topic
• Gene Amplification
• HLA-DR Antigens
• Histocompatibility Testing
• Humans
• Molecular Sequence Data