Article
Routine HLA DRB/DQB oligonucleotide typing by a non-radioactive dot-blot micromethod.
Journal of immunological methods - 13 Mar 1995
García-Pacheco J M, Mantilla P, García-Olivares E, Manzano-Fernández M N
Abstract excerpt
In the last few years the clinical need for HLA genotyping has become evident. However, the routine use of PCR-based DNA typing techniques has been hampered by economical and/or technical considerations. The classical PCR-SSO (product-dot) method has been widely tested and proven to be useful for large-scale HLA DNA typing. However, it is not a suitable method for routine typing of single samples because it takes...
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