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Article

Routine HLA DRB/DQB oligonucleotide typing by a non-radioactive dot-blot micromethod.

Journal of immunological methods - 13 Mar 1995

García-Pacheco J M, Mantilla P, García-Olivares E, Manzano-Fernández M N

Abstract excerpt

In the last few years the clinical need for HLA genotyping has become evident. However, the routine use of PCR-based DNA typing techniques has been hampered by economical and/or technical considerations. The classical PCR-SSO (product-dot) method has been widely tested and proven to be useful for large-scale HLA DNA typing. However, it is not a suitable method for routine typing of single samples because it takes...

Topics

Base Sequence
Cell Line
DNA
DNA Primers
DNA Probes
Female
Genotype
HLA-DQ Antigens
HLA-DR Antigens
Humans
Immunoblotting
Lymphocytes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Male

Molecular Sequence Data

Oligonucleotides

Polymerase Chain Reaction

Source

Identifiers

PMID: 7897246
DOI: 10.1016/0022-1759(94)00296-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7897246-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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