Article
Automation of large-scale HLA oligotyping using a robotic workstation.
BioTechniques - 1 Sept 1993
Caillat-Zucman S, Garchon H J, Costantino F, Cot S, Bach J F
Abstract excerpt
The ability to amplify specific genomic segments by the PCR has made the analysis of DNA polymorphism with oligonucleotidic probes a practical approach to HLA class II typing. However, to make this method more accurate and applicable to large-scale typing, technical and logistic limitations must be overcome and the risk of human errors must be reduced. To address this problem, we developed an automated procedure,...
Topics
- Alleles
- DNA
- Electrophoresis, Agar Gel
- Genes, MHC Class II
- Genotype
- Histocompatibility Antigens Class II
- Histocompatibility Testing
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Robotics