Article
Polymerase-chain-reaction-based analysis of polymorphism in the HLA-B gene.
Human immunology - 1 Aug 1992
Yoshida M, Kimura A, Numano F, Sasazuki T
Abstract excerpt
The polymerase chain reaction (PCR) in combination with the sequence-specific oligonucleotide probe (SSOP) was applied to analyze the polymorphism in the exon 2 of the HLA-B gene. In this study, genomic DNAs from 85 B-lymphoblastoid cell lines homozygous for HLA and peripheral blood granulocytes of 156 Japanese individuals were investigated. Two HLA-B-specific 5'-sided primers (CG4 and CG5) and two 3'-sided...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- DNA Probes
- Exons
- Granulocytes
- HLA-B Antigens
- Humans
- Japan
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
