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Article

Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP.

Acta medica Okayama - 1 Dec 1998

Inoue S, Yamamoto Y, Okamoto O, Murakami H, Miyaishi S, Ishizu H

Abstract excerpt

A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB generic primer pairs and the second round of PCRs (20 cycles) were performed using DRB1 group-specific primers. The...

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Source

Identifiers

PMID: 9876765
DOI: 10.18926/AMO/31305

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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