Article
Improvement of sensitivity in HLA-DRB1 typing by semi-nested PCR-RFLP.
Acta medica Okayama - 1 Dec 1998
Inoue S, Yamamoto Y, Okamoto O, Murakami H, Miyaishi S, Ishizu H
Abstract excerpt
A sensitive method of HLA-DRB1 typing was devised using a semi-nested polymerase chain reaction (PCR) followed by a restriction fragment length polymorphism (RFLP) analysis (semi-nested PCR-RFLP method). The first-round amplification (30 cycles) of the semi-nested PCR was performed using DRB gene...
Topics
- Genotype
- HLA-DR Antigens
- HLA-DRB1 Chains
- Humans
- Male
- Paternity
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sensitivity and Specificity