Article
Rapid HLA-DRB1 genotyping by nested PCR amplification.
Tissue antigens - 1 Feb 1992
Bein G, Gläser R, Kirchner H
Abstract excerpt
State of the art genotyping of HLA class II alleles with group-specific DNA amplification by the polymerase chain reaction (PCR) (1) and subsequent probing with sequence-specific oligonucleotides (2-4) is not suitable for typing cadaveric organ donors since the typing procedure takes far more than one working day. We designed specific oligonucleotide primer sets for nested PCR amplification which allowed typing...
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