Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid HLA-DRB1 genotyping by nested PCR amplification.

Tissue antigens - 1 Feb 1992

Bein G, Gläser R, Kirchner H

Abstract excerpt

State of the art genotyping of HLA class II alleles with group-specific DNA amplification by the polymerase chain reaction (PCR) (1) and subsequent probing with sequence-specific oligonucleotides (2-4) is not suitable for typing cadaveric organ donors since the typing procedure takes far more than one working day. We designed specific oligonucleotide primer sets for nested PCR amplification which allowed typing...

Topics

Alleles
Base Sequence
Blotting, Southern
DNA
Electrophoresis, Agar Gel
Exons
Gene Amplification
Genotype
HLA-DR Antigens
HLA-DR Serological Subtypes
HLA-DRB1 Chains
Histocompatibility Antigens Class II

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Molecular Sequence Data

Oligonucleotide Probes

Polymerase Chain Reaction

Source

Identifiers

PMID: 1574800
DOI: 10.1111/j.1399-0039.1992.tb01909.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1574800-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid HLA-DRB1 genotyping by nested PCR amplification.PMID 1574800Read ↗

Rapid HLA-DQB typing by eight polymerase chain reaction amplifications with sequence-specific primers (PCR-SSP).Semantic proximity Read ↗

HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation.Semantic proximity Read ↗

High resolution HLA-DRB1 SSP typing for cadaveric donor transplantation.Semantic proximity Read ↗

Identification of the HLA-DRB1*04, -DRB1*07, and -DRB1*09 alleles by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours.Semantic proximity Read ↗

Rapid DNA typing for class II HLA antigens: subtyping of DRw52-associated DRB1 alleles.Semantic proximity Read ↗

HLA class II genes: typing by DNA analysis.Semantic proximity Read ↗

Determination of DQB1 alleles using PCR amplification and allele-specific primers.Semantic proximity Read ↗

High-resolution HLA-DRB typing using denaturing gradient gel electrophoresis and direct sequencing.Semantic proximity Read ↗

High-throughput sequence-based typing strategy for HLA-DRB1 based on real-time polymerase chain reaction.Semantic proximity Read ↗

HLA-DOB1 "low-resolution' typing by PCR amplification with sequence-specific primers (PCR-SSP).Semantic proximity Read ↗

Determination of DRB alleles using PCR amplification and allele-specific primers.Semantic proximity Read ↗

HLA-DRB1*01 subtyping by allele-specific PCR amplification: a sensitive, specific and rapid technique.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.