Article
[HLA-DQ genotyping using a modified technique of PCR-RFLP: application to HLA-DQA1 gene].
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie - 1 Sept 1995
Haras D, Piperi M H, Cucchi-Mouillot P
Abstract excerpt
Since 1989, several HLA-DQA1 PCR-RFLP genotyping protocols have been published. These methods require complete digestion of the PCR products and determination of the restriction fragments length. The HLA-DQA1 PCR-RFLP genotyping protocol describe here uses one amplification step through PCR, digestion of the PCR-products with 8 restriction endonucleases, and determination of the fragments size after...
Topics
- Alleles
- DNA Restriction Enzymes
- Electrophoresis, Polyacrylamide Gel
- France
- Genotype
- HLA-DQ Antigens
- In Vitro Techniques
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length