Article
Enzyme-linked DNA oligotyping. A practical method for clinical HLA-DNA typing.
Human immunology - 1 Apr 1993
Lazaro A M, Fernandez-Viña M A, Liu Z, Stastny P
Abstract excerpt
The use of PCR and oligonucleotide hybridization has increased the accuracy and resolution of typing for HLA class II alleles, but current procedures, performed in batches, take too long and are not suited for testing single samples. We have developed a typing method using enzyme-linked oligonucl...
Topics
- Alkaline Phosphatase
- Alleles
- Base Sequence
- DNA
- Genes, MHC Class II
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Oligonucleotides
- Polymerase Chain Reaction