Article
Probing HLA-B7 conformational shifts induced by peptide-binding groove mutations and bound peptide with anti-HLA monoclonal antibodies.
Journal of immunology (Baltimore, Md. : 1950) - 15 Sept 1996
Smith K D, Mace B E, Valenzuela A, Vigna J L, McCutcheon J A, Barbosa J A, Huczko E, Engelhard V H, Lutz C T
Abstract excerpt
To determine the influence of peptide-binding groove residues and MHC-bound peptide on HLA-B7 conformation, we investigated the binding sites of nine locus- or allele-specific mAbs using a panel of 82 HLA-B7 variants. The functional mAb epitopes encircle the HLA-B7 peptide-binding groove. Three m...
Topics
- Antibodies, Monoclonal
- Binding, Competitive
- Cell Line
- Epitopes
- HLA-B7 Antigen
- Humans
- Mutation
- Peptide Fragments
- Protein Binding
- Protein Conformation
- Protein Folding