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Article

High-resolution HLA-DRB typing using denaturing gradient gel electrophoresis and direct sequencing.

Tissue antigens - 1 Aug 1997

Knapp L A, Lehmann E, Hennes L, Eberle M E, Watkins D I

Abstract excerpt

High-resolution HLA-DRB typing is required for bone marrow transplantation between unrelated donors and recipients and also for identification of novel HLA-DRB alleles. Here we describe a method for the unambiguous identification of HLA-DRB alleles using the polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct sequencing. The highly variable second exon of all HLA-DRB1,...

Topics

Alleles
DNA
DNA Primers
Electrophoresis, Polyacrylamide Gel
Exons
HLA-DR Antigens
Histocompatibility Testing
Humans
Nucleic Acid Denaturation
Polymerase Chain Reaction
Sequence Analysis, DNA

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Identifiers

PMID: 9271827
DOI: 10.1111/j.1399-0039.1997.tb02856.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-9271827-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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