HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.

doi:10.1007/BF00241635

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Article

HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.

Immunogenetics - 1 Jan 1990

Wordsworth B P, Allsopp C E, Young R P, Bell J I

Abstract excerpt

A series of sequence-specific oligonucleotides (SSOs) have been used to type alleles at the HLA-DRB1 locus. Genomic DNA was amplified to high copy number by the polymerase chain reaction (PCR) and hybridizations of the dot-blotted, amplified DNA to a series of 14 SSOs enabled the identification o...

Topics

Alleles
Base Sequence
Gene Amplification
HLA-DR Antigens
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction

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