Article
A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA.
Journal of molecular biology - 16 Aug 1996
Lázaro E, Rodriguez-Fonseca C, Porse B, Ureña D, Garrett R A, Ballesta J P
Abstract excerpt
Sparsomycin, a broad-spectrum antibiotic, acts at the peptidyl transferase centre of the ribosome, stabilizing peptidyl-tRNA binding at the P-site and weakening ternary complex binding. A sparsomycin-resistant mutant was isolated for the archaeon Halobacterium salinarium and shown to lack a post-...
Topics
- Anti-Bacterial Agents
- Base Sequence
- Binding Sites
- Halobacterium
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Peptidyl Transferases
- Protein Synthesis Inhibitors
- RNA Processing, Post-Transcriptional
- RNA, Ribosomal, 23S
- RNA, Transfer, Phe
- Ribosomes
- Sequence Analysis, RNA
- Sparsomycin
- Uridine