Article
Chemical, biochemical and genetic endeavours characterizing the interaction of sparsomycin with the ribosome.
Biochimie - 1 Jan 2000
Lazaro E, Van den Broek L A, San Felix A, Ottenheijm H C, Ballesta J P
Abstract excerpt
Sparsomycin interaction with the ribosome and characteristics of the drug binding site in the particle were studied using chemical modification of the drug, affinity labeling methods and isolation of drug resistant mutants. The structure-function relationship studies, performed with a large number of drug derivatives, indicate that the drug interacts with the ribosome by its western and eastern moieties. The...
Topics
- Binding Sites
- Drug Resistance, Microbial
- Halobacterium salinarum
- Mutation
- RNA, Bacterial
- RNA, Ribosomal
- Ribosomes
- Sparsomycin
- Structure-Activity Relationship