Article
Complete generic and extensive fine-specificity typing of the HLA-B locus by the PCR-SSOP method.
Tissue antigens - 1 Oct 1995
Fleischhauer K, Zino E, Bordignon C, Benazzi E
Abstract excerpt
This study describes sequence specific oligonucleotide probe (SSOP) typing of hypervariable regions in exons 2 and 3 of HLA-B locus genes. A single HLA-B specific PCR-product spanning from bp 84 in exon 2 to bp 241 in exon 3 was used for dot blot hybridization to forty-seven chemiluminescent labeled oligonucleotide probes. Thirty-one of these probes were derived from four hypervariability zones in exon 3 of HLA-B...
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