Article
Complete generic and extensive fine-specificity typing of the HLA-B locus by the PCR-SSOP method.
Tissue antigens - 1 Oct 1995
Fleischhauer K, Zino E, Bordignon C, Benazzi E
Abstract excerpt
This study describes sequence specific oligonucleotide probe (SSOP) typing of hypervariable regions in exons 2 and 3 of HLA-B locus genes. A single HLA-B specific PCR-product spanning from bp 84 in exon 2 to bp 241 in exon 3 was used for dot blot hybridization to forty-seven chemiluminescent labe...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cell Line, Transformed
- DNA Primers
- HLA-B Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Reproducibility of Results