Article
Ligation based HLA-B*27 typing.
Tissue antigens - 1 Sept 1996
Fischer G F, Faé I, Moser S, Petrasek M, Bässler H M, Menzel G R, Mayr W R
Abstract excerpt
We have established a ligation based typing method to detect HLA-B*27 alleles at the DNA level. The method requires amplification of exon 2 of the HLA-B locus from genomic DNA by the polymerase catalyzed chain reaction (PCR) using group specific primers. An aliquot of the PCR amplification product, heat stable ligase and a pair of oligonucleotide probes, designed to hybridize adjacently to HLA-B*27 specific...
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