Ligation based HLA-B*27 typing.

doi:10.1111/j.1399-0039.1996.tb02622.x

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Article

Ligation based HLA-B*27 typing.

Tissue antigens - 1 Sept 1996

Fischer G F, Faé I, Moser S, Petrasek M, Bässler H M, Menzel G R, Mayr W R

Abstract excerpt

We have established a ligation based typing method to detect HLA-B*27 alleles at the DNA level. The method requires amplification of exon 2 of the HLA-B locus from genomic DNA by the polymerase catalyzed chain reaction (PCR) using group specific primers. An aliquot of the PCR amplification produc...

Topics

Base Sequence
Genotype
HLA-B27 Antigen
Humans
Leukocytes, Mononuclear
Molecular Biology
Polymerase Chain Reaction

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