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Article

New high resolution typing strategy for HLA-A locus alleles based on dye terminator sequencing of haplotypic group-specific PCR-amplicons of exon 2 and exon 3.

Tissue antigens - 1 Jan 1999

Kurz B, Steiert I, Heuchert G, Müller C A

Abstract excerpt

In this study, a new sequencing-based typing strategy for the HLA-A locus is presented which involves group-specific separate amplification of exon 2 and 3 of HLA-A alleles in a first step. Conserved HLA-A locus-specific primers of intron 1 or 3 were combined in 10 primer-mixes with group-specifi...

Topics

Alleles
Base Sequence
Cell Line
DNA Primers
Deoxyribonucleotides
Exons
Fluorescent Dyes
Genes, MHC Class I
HLA-A Antigens
Haplotypes
Histocompatibility Testing
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Infant, Newborn

Molecular Sequence Data

Polymerase Chain Reaction

Reference Standards

Sequence Analysis, DNA

Source

Identifiers

PMID: 10082434
DOI: 10.1034/j.1399-0039.1999.530109.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-10082434-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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