Article
Reliable and fast allele-specific extension of 3'-LNA modified oligonucleotides covalently immobilized on a plastic base, combined with biotin-dUTP mediated optical detection.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Dec 2006
Michikawa Yuichi, Fujimoto Kentaro, Kinoshita Kenji, Kawai Seiko, Sugahara Keisuke, Suga Tomo, Otsuka Yoshimi, Fujiwara Kazuhiko, Iwakawa Mayumi, Imai Takashi
Abstract excerpt
In the present work, a convenient microarray SNP typing system has been developed using a plastic base that covalently immobilizes amino-modified oligonucleotides. Reliable SNP allele discrimination was achieved by using allelic specificity-enhanced enzymatic extension of immobilized oligonucleotide primer, with a locked nucleic acid (LNA) modification at the SNP-discriminating 3'-end nucleotide. Incorporation of...
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