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Article

Limited proteolysis and amino acid replacements in the effector region of Thermus thermophilus elongation factor Tu.

European journal of biochemistry - 15 Jul 1996

Zeidler W, Schirmer N K, Egle C, Ribeiro S, Kreutzer R, Sprinzl M

Abstract excerpt

The effector region of the elongation factor Tu (EF-Tu) from Thermus thermophilus was modified by limited proteolysis or via site-directed mutagenesis. The biochemical properties of the obtained EF-Tu variants were investigated with respect to partial reactions of the functional cycle of EF-Tu. EF-Tu that was cleaved at the Arg59-Gly60 peptide bond [EF-Tu-(1-59)/EF-Tu-(60-405)] bound GDP, EF-Ts and...

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Identifiers

PMID: 8706729
DOI: 10.1111/j.1432-1033.1996.0265u.x

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Limited proteolysis and amino acid replacements in the effector region of Thermus thermophilus elongation factor Tu.PMID 8706729Read ↗

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