Article
Limited proteolysis and amino acid replacements in the effector region of Thermus thermophilus elongation factor Tu.
European journal of biochemistry - 15 Jul 1996
Zeidler W, Schirmer N K, Egle C, Ribeiro S, Kreutzer R, Sprinzl M
Abstract excerpt
The effector region of the elongation factor Tu (EF-Tu) from Thermus thermophilus was modified by limited proteolysis or via site-directed mutagenesis. The biochemical properties of the obtained EF-Tu variants were investigated with respect to partial reactions of the functional cycle of EF-Tu. E...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Endopeptidases
- Escherichia coli
- GTP Phosphohydrolase-Linked Elongation Factors
- Genetic Variation
- Guanosine Diphosphate
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Peptide Biosynthesis
- Peptide Elongation Factor Tu
- Peptide Mapping
- Peptides
- Point Mutation