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Article

Design and properties of efficient tRNA:EF-Tu FRET system for studies of ribosomal translation.

Protein engineering, design & selection : PEDS - 1 May 2013

Chudaev Maxim, Poruri Kiran, Goldman Emanuel, Jakubowski Hieronim, Jain Mohit Raja, Chen Wei, Li Hong, Tyagi Sanjay, Mandecki Wlodek

Abstract excerpt

Formation of the ternary complex between GTP-bound form of elongation factor Tu (EF-Tu) and aminoacylated transfer RNA (aa-tRNA) is a key event in protein biosynthesis. Here we show that fluorescently modified Escherichia coli EF-Tu carrying three mutations, C137A, C255V and E348C, and fluorescently modified Phe-tRNA(Phe) form functionally active ternary complex that has properties similar to those of the...

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Identifiers

PMID: 23447652
DOI: 10.1093/protein/gzt006
PMCID: PMC3630515

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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