Article
The influence of different modifications of elongation factor Tu from Escherichia coli on ternary complex formation investigated by fluorescence spectroscopy.
Nucleic acids research - 11 Feb 1990
Ott G, Jonák J, Abrahams I P, Sprinzl M
Abstract excerpt
A fluorescence titration assay was used to detect the effects of various modifications of E.coli elongation factor Tu on the formation of the ternary complex with aminoacyl-tRNAs. The treatment of EF-Tu.GDP with TPCK, an analogue of the 3'terminus of aminoacyl-tRNA, was found to have no influence on the conversion of EF-Tu.GDP to 'active' EF-Tu.GTP, but does decrease the affinity of the activated protein for...
Topics
- Escherichia coli
- Guanosine Diphosphate
- Guanosine Triphosphate
- Mutation
- Peptide Elongation Factor Tu
- RNA, Transfer, Amino Acyl
- RNA, Transfer, Phe
- RNA, Transfer, Tyr
- Spectrometry, Fluorescence
- Tosylphenylalanyl Chloromethyl Ketone