Article
A rapid screen of active site mutants in glycinamide ribonucleotide transformylase.
Biochemistry - 9 Jul 1996
Warren M S, Marolewski A E, Benkovic S J
Abstract excerpt
Specific and saturation site-directed mutageneses have been used to alter each polar residue within 6 A of the catalytic center of glycinamide ribonucleotide transformylase (EC 2.1.2.2). These mutants were rapidly screened for catalytic activity using functional complementation of auxotrophic cel...
Topics
- Acyltransferases
- Binding Sites
- Catalysis
- Escherichia coli
- Genetic Complementation Test
- Hydrogen Bonding
- Hydroxymethyl and Formyl Transferases
- Models, Chemical
- Mutagenesis, Site-Directed
- Mutation
- Phosphoribosylglycinamide Formyltransferase
- Protein Engineering
- Selection, Genetic
- Sequence Analysis, DNA
- Structure-Activity Relationship