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Article

Essential role of an active-site guanine in glmS ribozyme catalysis.

Journal of the American Chemical Society - 5 Dec 2007

Klein Daniel J, Been Michael D, Ferré-D'Amaré Adrian R

Abstract excerpt

The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile...

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Identifiers

PMID: 17990888
DOI: 10.1021/ja0768441

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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