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Article

The role of Glu73 of barnase in catalysis and the binding of barstar.

Journal of molecular biology - 4 Jul 1997

Schreiber G, Frisch C, Fersht A R

Abstract excerpt

Barnase, a small extracellular ribonuclease from Bacillus amyloliquefaciens and its intracellular inhibitor barstar have co-evolved to bind tightly and rapidly. Barnase has also evolved to be catalytically active. The active site of barnase and its binding site for barstar use the same subset of amino acids. The exception is Glu73 (the general base in catalysis), which although located at the centre of the...

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Identifiers

PMID: 9231905
DOI: 10.1006/jmbi.1997.1080

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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The role of Glu73 of barnase in catalysis and the binding of barstar.PMID 9231905Read ↗

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Recognition between a bacterial ribonuclease, barnase, and its natural inhibitor, barstar.Semantic proximity Read ↗

How optimal are the binding energetics of barnase and barstar?Semantic proximity Read ↗

Experimental assignment of the structure of the transition state for the association of barnase and barstar.Semantic proximity Read ↗

Thermodynamics of the interaction of barnase and barstar: changes in free energy versus changes in enthalpy on mutation.Semantic proximity Read ↗

[I87E mutation prevents barstar dimerization].Semantic proximity Read ↗

Active barnase variants with completely random hydrophobic cores.Semantic proximity Read ↗

A structural double-mutant cycle: estimating the strength of a buried salt bridge in barnase.Semantic proximity Read ↗

Structural and chemical basis for glucosamine 6-phosphate binding and activation of the glmS ribozyme.Semantic proximity Read ↗

Substitution of an essential adenine in the U1A-RNA complex with a non-polar isostere.Semantic proximity Read ↗

A calorimetric study of the thermal stability of barstar and its interaction with barnase.Semantic proximity Read ↗

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